Siva Rama Raju Kanumuri

Siva Rama Raju Kanumuri, Ph.D.

Assistant Scientist

Department: Pharmaceutics
Business Phone: (513) 344-0547
Business Email:

About Siva Rama Raju Kanumuri

Siva Rama Raju Kanumuri is an Assistant Scientist in Department of Pharmaceutics and CTSI-Translational Drug Development Core at University of Florida, Gainesville. He received his bachelor’s degree in Pharmaceutical Sciences from Andhra University, India in 2008 and later received his Master of Science (M.S.) in Pharmaceutics from National Institute of Pharmaceutical Education and Research, Raebareli, India in 2011 with a MHRD-Post-graduate fellowship by All India Council for Technical Education (AICTE), India. After his Master’s, he worked as a project associate for 2 years in CSIR- Central Drug Research Institute, India and later joined as CSIR- Senior Research Fellowship (2013-2016) to pursue graduation (Ph.D.) from the same institute. After his graduate studies, Dr. Raju moved to US and joined as a postdoctoral fellow under the supervision of Professor Giovanni M. Pauletti at the University of Cincinnati (2017-2019) and later under the supervision of Dr. Christopher McCurdy at the University of Florida (2019-2022).


International Travel Award
2015 · Centre for International Co-operation in Science (India)
International Travel Award to attend AAPS Conference
2015 · Department of Science and Technology, New Delhi (India)
ISSX Travel Award
2015 · International Society for the Study of Xenobiotics (ISSX) Organizing committee
CSIR- Senior Research Fellowship
2013-2016 · Council for Scientific and Industrial Research (CSIR), India
8th Dr. P.D. Sethi Annual Award 2012 for Best Paper in Pharmaceutical Analysis
2012 · Anchrom Pvt Ltd (India)
Silver Medal in M.S (Pharmaceutics)
2011 · National Institute of Pharmaceutical Education and Research (NIPER), Raebareli, India
MHRD-Post-graduate fellowship
2009-2011 · All India Council for Technical Education, India

Research Profile

Dr. Siva Rama Raju Kanumuri is a pharmacokinetics scientist with more than 10 years of research experience in small molecule preclinical discovery ADMET to clinical pharmacokinetic studies. His research work focusses on designing and orchestrating the execution of various in vitro and in vivo pharmacokinetic studies (human, rodents, and non-rodents) for optimization of ADMET properties of new chemical entities (NCE), drugs under development and natural products. His research helps identify small molecules to move forward in drug development based on metabolic stability, metabolite identification, formation of reactive metabolites and drug transport and permeability across biological barriers.

Open Researcher and Contributor ID (ORCID)


Areas of Interest
  • Bioanalysis
  • Drug Metabolism
  • PK-PD Modelling
  • Pharmacokinetic Modeling and Simulation
  • Pharmacokinetics/pharmacodynamics
  • Population pharmacokinetics


DR5 disulfide bonding as a sensor and effector of protein folding stress.
bioRxiv : the preprint server for biology. [DOI] 10.1101/2024.03.04.583390. [PMID] 38496520.
Effects of kratom on driving: Results from a cross-sectional survey, ecological momentary assessment, and pilot simulated driving Study.
Traffic injury prevention. 25(4):594-603 [DOI] 10.1080/15389588.2024.2327827. [PMID] 38497810.
Explorations of Agonist Selectivity for the α9* nAChR with Novel Substituted Carbamoyl/Amido/Heteroaryl Dialkylpiperazinium Salts and Their Therapeutic Implications in Pain and Inflammation.
Journal of medicinal chemistry. 67(11):8642-8666 [DOI] 10.1021/acs.jmedchem.3c02429. [PMID] 38748608.
Pharmacokinetic Interaction of Kratom and Cannabidiol in Male Rats
Pharmaceutics. 16(3) [DOI] 10.3390/pharmaceutics16030318. [PMID] 38543213.
Preclinical pharmacokinetic studies of villocarine A, an active Uncaria alkaloid.
Drug testing and analysis. [DOI] 10.1002/dta.3703. [PMID] 38747129.
Responses to a “Typical” Morning Dose of Kratom in People Who Use Kratom Regularly: A Direct-Observation Study
Journal of Addiction Medicine. 18(2):144-152 [DOI] 10.1097/adm.0000000000001259.
An in vitro evaluation of kratom (Mitragyna speciosa) on the catalytic activity of carboxylesterase 1 (CES1)
Chemico-Biological Interactions. 384 [DOI] 10.1016/j.cbi.2023.110715. [PMID] 37716419.
Comparative Pharmacokinetics of Commercially Available Cannabidiol Isolate, Broad-Spectrum, and Full-Spectrum Products.
European journal of drug metabolism and pharmacokinetics. 48(4):427-435 [DOI] 10.1007/s13318-023-00839-3. [PMID] 37337087.
Metabolite and Molecular Characterization of Mitragyna speciosa Identifies Developmental and Genotypic Effects on Monoterpene Indole and Oxindole Alkaloid Composition
Journal of Natural Products. 86(4):1042-1052 [DOI] 10.1021/acs.jnatprod.3c00092.
Oral IRAK-4 Inhibitor CA-4948 Is Blood-Brain Barrier Penetrant and Has Single-Agent Activity against CNS Lymphoma and Melanoma Brain Metastases
Clinical Cancer Research. 29(9):1751-1762 [DOI] 10.1158/1078-0432.ccr-22-1682.
Pharmacokinetic and Pharmacodynamic Consequences of Cytochrome P450 3A Inhibition on Mitragynine Metabolism in Rats
Journal of Pharmacology and Experimental Therapeutics. 385(3):180-192 [DOI] 10.1124/jpet.122.001525. [PMID] 37019472.
Clinical pharmacokinetics of kavalactones after oral dosing of standardized kava extract in healthy volunteers
Journal of Ethnopharmacology. 297 [DOI] 10.1016/j.jep.2022.115514. [PMID] 35777607.
Metabolism of Speciociliatine, an Overlooked Kratom Alkaloid for its Potential Pharmacological Effects.
The AAPS journal. 24(5) [DOI] 10.1208/s12248-022-00736-8. [PMID] 35854066.
The Lack of Contribution of 7-Hydroxymitragynine to the Antinociceptive Effects of Mitragynine in Mice: A Pharmacokinetic and Pharmacodynamic Study
Drug Metabolism and Disposition. 50(2):158-167 [DOI] 10.1124/dmd.121.000640. [PMID] 34759012.
UPLC-MS/MS method for the quantification of MCI-77, a novel sigma-1 receptor ligand, and its application to pharmacokinetic studies
Journal of Chromatography B. 1196 [DOI] 10.1016/j.jchromb.2022.123187.
A novel nanosized phospholipid complex of Biochanin A for improving oral bioavailability: Preparation and in-vitro/in-vivo characterizations
Journal of Drug Delivery Science and Technology. 61 [DOI] 10.1016/j.jddst.2020.102254.
Characterization of different forms of kava (Piper methysticum) products by UPLC-MS/MS.
Planta medica. 88(14):1348-1359 [DOI] 10.1055/a-1708-1994. [PMID] 34839465.
Chromatographic separation and estimation of natural antimalarial flavonoids in biological matrices
Proceedings of the Indian National Science Academy. 87(3):446-468 [DOI] 10.1007/s43538-021-00050-5.
Elucidation of plasma protein binding, blood partitioning, permeability, CYP phenotyping and CYP inhibition studies of Withanone using validated UPLC method: An active constituent of neuroprotective herb Ashwagandha.
Journal of ethnopharmacology. 270 [DOI] 10.1016/j.jep.2021.113819. [PMID] 33460762.
Exploring the Chemistry of Alkaloids from Malaysian Mitragyna speciosa (Kratom) and the Role of Oxindoles on Human Opioid Receptors.
Journal of natural products. 84(4):1034-1043 [DOI] 10.1021/acs.jnatprod.0c01055. [PMID] 33635670.
Highly Specific Sigma Receptor Ligands Exhibit Anti-Viral Properties in SARS-CoV-2 Infected Cells
Pathogens. 10(11) [DOI] 10.3390/pathogens10111514. [PMID] 34832669.
Oral Pharmacokinetics in Beagle Dogs of the Mitragynine Metabolite, 7-Hydroxymitragynine.
European journal of drug metabolism and pharmacokinetics. 46(3):459-463 [DOI] 10.1007/s13318-021-00684-2. [PMID] 33847897.
Preclinical pharmacokinetic study of speciociliatine, a kratom alkaloid, in rats using an UPLC-MS/MS method.
Journal of pharmaceutical and biomedical analysis. 194 [DOI] 10.1016/j.jpba.2020.113778. [PMID] 33277117.
Bioanalytical method development and pharmacokinetics of MCI-92, a sigma-1 receptor ligand.
Journal of pharmaceutical and biomedical analysis. 191 [DOI] 10.1016/j.jpba.2020.113610. [PMID] 32971495.
Bioavailability, tissue distribution and excretion studies of a potential anti-osteoporotic agent, medicarpin, in female rats using validated LC-MS/MS method.
Journal of pharmaceutical and biomedical analysis. 180 [DOI] 10.1016/j.jpba.2019.112978. [PMID] 31855725.
Exploration of cytochrome P450 inhibition mediated drug-drug interaction potential of kratom alkaloids.
Toxicology letters. 319:148-154 [DOI] 10.1016/j.toxlet.2019.11.005. [PMID] 31707106.
Metabolism of a Kratom Alkaloid Metabolite in Human Plasma Increases Its Opioid Potency and Efficacy.
ACS pharmacology & translational science. 3(6):1063-1068 [DOI] 10.1021/acsptsci.0c00075. [PMID] 33344889.
Pharmacokinetics and Safety of Mitragynine in Beagle Dogs.
Planta medica. 86(17):1278-1285 [DOI] 10.1055/a-1212-5475. [PMID] 32693425.
Regulatory sampling of industrial hemp plant samples (Cannabis sativa L.) using UPLC-MS/MS method for detection and quantification of twelve cannabinoids.
Journal of cannabis research. 2(1) [DOI] 10.1186/s42238-020-00050-0. [PMID] 33526142.
LC-ESI-MS/MS method for the simultaneous determination of isoformononetin, daidzein, and equol in rat plasma: Application to a preclinical pharmacokinetic study
Journal of Chromatography B. 1129 [DOI] 10.1016/j.jchromb.2019.121776.
Development and validation of UPLC-MS/MS assay for quantification of cladrin: Absolute bioavailability and dose proportionality study in rats
Journal of Pharmaceutical and Biomedical Analysis. 152:289-297 [DOI] 10.1016/j.jpba.2018.01.044.
Metabolic Enhancer Piracetam Attenuates the Translocation of Mitochondrion-Specific Proteins of Caspase-Independent Pathway, Poly [ADP-Ribose] Polymerase 1 Up-regulation and Oxidative DNA Fragmentation
Neurotoxicity Research. 34(2):198-219 [DOI] 10.1007/s12640-018-9878-2.
New therapeutic activity of metabolic enhancer piracetam in treatment of neurodegenerative disease: Participation of caspase independent death factors, oxidative stress, inflammatory responses and apoptosis
Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease. 1864(6):2078-2096 [DOI] 10.1016/j.bbadis.2018.03.014.
Species differences between rat and human in vitro metabolite profile, in vivo predicted clearance, CYP450 inhibition and CYP450 isoforms that metabolize benzanthrone: Implications in risk assessment
Food and Chemical Toxicology. 111:94-101 [DOI] 10.1016/j.fct.2017.11.009.
Antileishmanial Activity of Pyrazolopyridine Derivatives and Their Potential as an Adjunct Therapy with Miltefosine.
Journal of medicinal chemistry. 60(3):1041-1059 [DOI] 10.1021/acs.jmedchem.6b01447. [PMID] 28059524.
Bioanalysis of antitubercular drugs using liquid chromatography.
Journal of pharmaceutical and biomedical analysis. 134:295-309 [DOI] 10.1016/j.jpba.2016.11.006. [PMID] 27951471.
Pharmacological activation of aldehyde dehydrogenase 2 promotes osteoblast differentiation via bone morphogenetic protein-2 and induces bone anabolic effect
Toxicology and Applied Pharmacology. 316:63-73 [DOI] 10.1016/j.taap.2016.12.013.
RP-HPLC Separation of Isomeric Withanolides: Method Development, Validation and Application to In situ Rat Permeability Determination
Journal of Chromatographic Science. 55(7):729-735 [DOI] 10.1093/chromsci/bmx027.
Synthesis and Evaluation of Chirally Defined Side Chain Variants of 7-Chloro-4-Aminoquinoline To Overcome Drug Resistance in Malaria Chemotherapy
Antimicrobial Agents and Chemotherapy. 61(3) [DOI] 10.1128/aac.01152-16.
Comparative assessment of bioanalytical method validation guidelines for pharmaceutical industry
Journal of Pharmaceutical and Biomedical Analysis. 126:83-97 [DOI] 10.1016/j.jpba.2016.03.052.
DBS-platform for biomonitoring and toxicokinetics of toxicants: proof of concept using LC-MS/MS analysis of fipronil and its metabolites in blood
Scientific Reports. 6(1) [DOI] 10.1038/srep22447. [PMID] 26960908.
Development and validation of an LC-MS/MS method for simultaneous determination of piperaquine and 97-63, the active metabolite of CDRI 97-78, in rat plasma and its application in interaction study
Drug Testing and Analysis. 8(2):221-227 [DOI] 10.1002/dta.1807.
Dietary Isoflavones as Modulators of Drug Metabolizing Enzymes and Transporters: Effect on Prescription Medicines
Critical Reviews in Food Science and Nutrition. 56(sup1):S95-S109 [DOI] 10.1080/10408398.2015.1045968.
Effect of polymorphism on the preclinical pharmacoknetics of desbutyl-lumefantrine, a potential anti-malarial agent
Drug Metabolism Reviews. 48((Suppl 1))
Naturally Occurring Carbazole Alkaloids from Murraya koenigii as Potential Antidiabetic Agents.
Journal of natural products. 79(5):1276-84 [DOI] 10.1021/acs.jnatprod.5b00883. [PMID] 27136692.
Pharmacokinetic endeavors for antimalarial therapeutics
International Journal of Infectious Diseases. 45:130-131 [DOI] 10.1016/j.ijid.2016.02.323.
Preclinical pharmacokinetics and species differences in the CYP-mediated metabolism of isoformononetin, a potential anti-osteoporotic isoflavonoid
Drug Metabolism Reviews. 48(Suppl 1) [DOI] 10.3109/03602532.2015.107193.
Validation of a Rapid and Sensitive UPLC–MS-MS Method Coupled with Protein Precipitation for the Simultaneous Determination of Seven Pyrethroids in 100 µL of Rat Plasma by Using Ammonium Adduct as Precursor Ion
Journal of Analytical Toxicology. 40(3):213-221 [DOI] 10.1093/jat/bkw002. [PMID] 26801239.
Arteether nanoemulsion for enhanced efficacy against Plasmodium yoelii nigeriensis malaria: An approach by enhanced bioavailability
Colloids and Surfaces B: Biointerfaces. 126:467-475 [DOI] 10.1016/j.colsurfb.2014.12.052.
Assessment of pharmacokinetic compatibility of short acting CDRI candidate trioxane derivative, 99–411, with long acting prescription antimalarials, lumefantrine and piperaquine
Scientific Reports. 5(1) [DOI] 10.1038/srep17264. [PMID] 26602250.
Bioavailability, plasma protein binding and metabolic stability studies of a ALDH2 activator, alda-1, using a validated LC-ESI-MS/MS method in rat plasma
RSC Advances. 5(67):54395-54402 [DOI] 10.1039/c5ra06859b.
Dried blood spots for neonates and pediatric populations
New Sampling Strategies in Toxicology and Therapeutic Drug Monitoring. 54-65 [DOI] 10.4155/fseb2013.14.363.
In vivo prediction of CYP-mediated metabolic interaction potential of formononetin and biochanin A using in vitro human and rat CYP450 inhibition data
Toxicology Letters. 239(1):1-8 [DOI] 10.1016/j.toxlet.2015.08.202.
LC-ESI–MS/MS method for bioanalytical determination of osteogenic phytoalexin, medicarpin, and its application to preliminary pharmacokinetic studies in rats
Journal of Chromatography B. 1001:9-16 [DOI] 10.1016/j.jchromb.2015.06.025.
Metabolic enhancer piracetam attenuates rotenone induced oxidative stress: a study in different rat brain regions.
Acta neurobiologiae experimentalis. 75(4):399-411 [DOI] 10.55782/ane-2015-2045. [PMID] 26994419.
No effect on pharmacokinetics of tamoxifen and 4-hydroxytamoxifen by multiple doses of red clover capsule in rats
Scientific Reports. 5(1) [DOI] 10.1038/srep16126. [PMID] 26530625.
Phytochemical analysis of isoflavonoids using liquid chromatography coupled with tandem mass spectrometry
Phytochemistry Reviews. 14(3):469-498 [DOI] 10.1007/s11101-015-9400-x.
Simultaneous determination of centchroman and tamoxifen along with their metabolites in rat plasma using LC–MS/MS
Bioanalysis. 7(8):967-979 [DOI] 10.4155/bio.14.253.
Simultaneous quantification of proposed anti-malarial combination comprising of lumefantrine and CDRI 97–78 in rat plasma using the HPLC–ESI-MS/MS method: application to drug interaction study
Malaria Journal. 14(1) [DOI] 10.1186/s12936-015-0684-5. [PMID] 25895956.
Effect of Red Clover on CYP Expression: An Investigation of Herb-Drug Interaction at Molecular Level.
Indian journal of pharmaceutical sciences. 76(3):261-6 [PMID] 25035541.
Investigation of the Functional Role of P-Glycoprotein in Limiting the Oral Bioavailability of Lumefantrine
Antimicrobial Agents and Chemotherapy. 58(1):489-494 [DOI] 10.1128/aac.01382-13.
Palladium and copper-catalyzed ligand-free coupling of phenylhydrazines in water
RSC Adv.. 4(82):43336-43340 [DOI] 10.1039/c4ra04621h.
Pharmacokinetics study of arteether loaded solid lipid nanoparticles: An improved oral bioavailability in rats
International Journal of Pharmaceutics. 466(1-2):321-327 [DOI] 10.1016/j.ijpharm.2014.03.036.
Self-nanoemulsifying drug delivery systems (SNEDDS) for oral delivery of arteether: pharmacokinetics, toxicity and antimalarial activity in mice
RSC Adv.. 4(110):64905-64918 [DOI] 10.1039/c4ra09267h.
The metabolic enhancer piracetam attenuates mitochondrion-specific endonuclease G translocation and oxidative DNA fragmentation
Free Radical Biology and Medicine. 73:278-290 [DOI] 10.1016/j.freeradbiomed.2014.05.014.
Bioanalysis of antimalarials using liquid chromatography
TrAC Trends in Analytical Chemistry. 42:186-204 [DOI] 10.1016/j.trac.2012.09.014.
Disposition of Pharmacologically Active Dietary Isoflavones in Biological Systems
Current Drug Metabolism. 14(4):369-380 [DOI] 10.2174/1389200211314040002.
Dried blood spots in bioanalysis of antimalarials: relevance and challenges in quantitative assessment of antimalarial drugs
Bioanalysis. 5(17):2171-2186 [DOI] 10.4155/bio.13.180.
Nanotechnology in Modern Medical Imaging and Interventions
Book Chapter.
Synthesis and Antimalarial Activity of 3,3-Spiroanellated 5,6-Disubstituted 1,2,4-Trioxanes
ACS Medicinal Chemistry Letters. 4(2):165-169 [DOI] 10.1021/ml300188t. [PMID] 24900640.
Synthesis and insight into the structure-activity relationships of chalcones as antimalarial agents.
Journal of medicinal chemistry. 56(1):31-45 [DOI] 10.1021/jm300588j. [PMID] 23270565.
Utility of noninvasive biomatrices in pharmacokinetic studies
Biomedical Chromatography. 27(10):1354-1366 [DOI] 10.1002/bmc.2996.
Fate of pharmacologically active isoflavones upon administration in sprague dawley rats
J Bioavailability and Bioequivalence. 4(3)
Reduced Bioavailability of Tamoxifen and its Metabolite 4-Hydroxytamoxifen After Oral Administration with Biochanin A (an Isoflavone) in Rats
Phytotherapy Research. 26(2):303-307 [DOI] 10.1002/ptr.3652.
Simultaneous determination of nine model compounds in permeability samples using RP-HPLC: Application to prove the cassette administration principle in single pass intestinal perfusion study in rats
Journal of Pharmaceutical and Biomedical Analysis. 67-68:71-76 [DOI] 10.1016/j.jpba.2012.03.048.
Intravenous pharmacokinetics, oral bioavailability, dose proportionality and in situ permeability of anti-malarial lumefantrine in rats
Malaria Journal. 10(1) [DOI] 10.1186/1475-2875-10-293. [PMID] 21985153.
Comparative study on various tableting properties of Phenytoin sodium mouth dissolving dosage form with and without spherical crystals
Int J of Pharm Sci Tech. 5(2):19-26
Nanosuspension: an attempt to enhance bioavailability of poorly soluble drugs
International Journal of Pharmaceutical Sciences and Research. 1(9):1-11
Int J Pharm Sci Rev Res. 4(3)


PG Diploma in Patent Law
2014-2015 · NALSAR University of Law, India
2013-2016 · CSIR-Central Drug Research Institute/ Academy of Scientific and Innovative Research, India
M.S. (Pharmaceutics)
2009-2011 · Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER), India
Bachelor of Pharmacy
2004-2008 · Andhra University, India

Contact Details

(513) 344-0547
Business Mailing:
PO Box 100494